Thp1 itaconate

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August undefined, 2024

WebApr 29, 2024 · Background Inflammatory stimuli induce immunoresponsive gene 1 (IRG1) expression that in turn catalyzes the production of itaconate from the tricarboxylic acid cycle. Itaconate has recently emerged as a regulator of immune cell functions, especially in macrophages. Studies show that itaconate is required for the activation of anti …

The role of itaconate in host defense and inflammation - PubMed

WebJul 15, 2024 · (A–D) RAW264.7 and THP1 cells were treated with LPS (50-5000 ng/mL) in the absence or presence of 3′3′-cGAMP (10 μg/mL) for 6 h, and then Acod1/ACOD1 mRNA … WebMar 24, 2024 · Itaconate is a mitochondrial metabolite, produced in high amounts by macrophages and monocytes of mice and humans upon activation by several inflammatory stimuli. In 2013, the identity of the itaconate-producing enzyme was revealed as aconitate decarboxylase 1, encoded by the gene Acod1 (previously known as immune-responsive … penny sheldon reddit

Metabolic reprogramming in macrophage responses

WebJun 19, 2024 · Itaconate production was the only metabolic pathway we tested that was capable of significantly regulating tumor burden. Irg1 expression has been reported in several cell types ( 24 , 38 – 40 ), but in the peritoneal cavity, it is Irg1 expression by resident Mϕ that promotes tumor growth. WebJun 4, 2024 · Itaconate is an anti-inflammatory metabolite involved in pathogen–macrophage interactions, but the mechanisms underlying its effect are not … WebJun 4, 2024 · Itaconate is an anti-inflammatory metabolite involved in pathogen–macrophage interactions, but the mechanisms underlying its effect are not fully understood. Competitive cysteine profiling has been performed to interrogate itaconate’s reactivity in cell lysates, but methods for analyzing targets of itaconation directly in living … toby pennock excavation

Itaconic acid induces ferroptosis by activating ferritinophagy

Staphylococcus aureus induces an itaconate-dominated …

To evaluate the immunometabolic response to Gram-positive bacteria we infected mice intranasally with S. aureus (USA300-LAC) and performed metabolomics on the bronchoalveolar lavage (BAL) fluid. As a positive control, we used an LPS-expressing Pseudomonas aeruginosa strain (PAO1)10. We observed that S. … See more The metabolic profile preferred by S. aureus at specific sites of infection directs the type of immune response deployed by the host33,34. We postulated that the … See more While itaconate stimulates anti-inflammatory and anti-oxidant responses in host cells, it is also bactericidal against S. aureus16. S. aureus responds to adverse … See more Reduced protein synthesis and growth despite an abundance of carbon sources in the environment suggested that itaconate-treated S. aureus had impaired … See more To evaluate the clinical relevance of these itaconate-induced metabolic changes, we studied a longitudinal collection of S. aureus strains isolated over 15 years from … See more WebItaconic acid, or methylidenesuccinic acid, is an organic compound. This dicarboxylic acid is a white solid that is soluble in water, ethanol, and acetone. Historically, itaconic acid was obtained by the distillation of citric acid, but currently it is produced by fermentation. The name itaconic acid was devised as an anagram of aconitic acid ... toby pentecost candourWeb生物医学研究院叶丹团队在《自然—细胞生物学》揭示免疫代谢物衣康酸特异性抑制TET双加氧酶衣康酸（Itaconate,ITA）是近年来在巨噬细胞中发现的具有显著抗炎活性的小分子代 … toby penty ranking

"WebFeb 29, 2024 · Using LC–MS metabolomics, Mills et al. have shown that itaconate is an anti-inflammatory metabolites which has important role in regulation of macrophage functions (Mills et al. 2024). However, Rattigan et al. demonstrated a complex role of itaconate as an inflammatory and anti-inflammatory key metabolite (Rattigan et al. 2024). " - Thp1 itaconate

Thp1 itaconate

Dimethyl itaconate, an itaconate derivative, exhibits …

WebTHP1 and immortalized bone-marrow-derived macrophages (iBMDM) with 4-octyl itaconate (OI), a cell-permeable itaconate derivative, induced efﬁcient translocation of TFEB from … WebMar 28, 2024 · Itaconate is produced by IRG1 catalyzing the TCA cycle intermediate cis-aconitate in the mitochondrial matrix, RAW264.7 macrophages were stimulated with DMXAA for 4 h and 6 h, and IRG1 was significantly increased (Figure 1 D).Similarly, THP1 cells were stimulated with 2′,3′cGAMP (human STING agonist) for 6 h, and IRG1 was significantly up …

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WebJan 6, 2024 · Bambouskova et al. demonstrated that due to their electrophilic properties, itaconate and its derivatives react with glutathione and induce both Nrf2-dependent and Nrf2-independent responses; moreover, they showed that the electrophilic stress caused by itaconate can regulate the IL-6 response, which is a regulatory secondary transcriptional … WebAug 4, 2024 · Zhang et al. showed that treatment of THP1 and immortalized bone-marrow-derived macrophages (iBMDM) with 4-octyl itaconate (OI), a cell-permeable itaconate …

WebJun 2, 2024 · Metrics. Itaconate is an immunomodulatory Krebs cycle-derived metabolite. Description of two isomers of itaconate, mesaconate and citraconate, which have … WebMay 12, 2024 · Metabolites have recently been found to be involved in significant biological regulation and changes. Itaconate, an important intermediate metabolite isolated from the tricarboxylic acid cycle, is derived from cis-aconitate decarboxylation mediated by immune response gene 1 in mitochondrial matrix. Itaconate has emerged as a key autocrine …

WebApr 29, 2024 · Background Inflammatory stimuli induce immunoresponsive gene 1 (IRG1) expression that in turn catalyzes the production of itaconate from the tricarboxylic acid … WebOne of the first reported inhibitors of ICL1 was itaconate , 14–16 a mammalian antimicrobial metabolite that is upregulated in lipopolysaccharide activated macrophages. 17–20 Itaconate is an α,β-unsaturated dicarboxylic acid that is structurally analogous to succinate, a product of the ICL-catalysed reaction .

WebOct 20, 2024 · Itaconate is produced from the mitochondrial TCA cycle enzyme aconitase decarboxylase (encoded by immune responsive gene1; Irg1) that exerts immunomodulatory function in myeloid cells. However ...

WebDec 17, 2024 · To determine the impact of itaconic acid in cell death, we treated THP1 cells (a human monocytic cell line widely used in itaconic acid metabolism research [10 ... has emerged as a regulator of immunometabolism that operates by producing metabolite itaconate. Here, we report a key role of STING1 (also known as STING and TMEM173 ... toby pepper insuranceWebAbstract. Itaconate is an immunoregulatory and anti-bacterial metabolite, and plays important roles in host–pathogen interactions. Chemoproteomic strategies have been used to explore the anti-inflammatory effects of itaconate on activated macrophages and it has been found that many key proteins in immune pathways were modified; however, how … toby penty olympicsWebJan 18, 2024 · Itaconate and its derivatives have antiinflammatory effects in preclinical models of sepsis, viral infections, psoriasis, gout, ischemia/reperfusion injury, and … toby pennington exeterWebMar 28, 2024 · Itaconate alkylates cysteine residues 151, 257, 288, 273 and 297 on the protein KEAP1, enabling Nrf2 to increase the expression of downstream genes with anti-oxidant and anti-inflammatory ... toby percivalWebDec 17, 2024 · After pro-inflammatory activation, the concentration of itaconic acid can be elevated to millimolar level (2–8 mM) [16, 17].To determine the impact of itaconic acid in … penny sheldonWebSep 21, 2024 · Recently, some studies have shown that immune responsive gene 1 (IRG1, also called ACOD1) was upregulated significantly by IFN-γ or LPS stimuli. 6 IRG1 is an … toby percival cheerleaderWebHowever, V155M-THP1 cells became more sensitive to LPS-induced ACOD1 expression (Figure 1I) and itaconate production (Figure 1J). Importantly, LPS-induced ACOD1 protein expression and itaconate production were blocked in STING1 −/− THP1 cells and this phenotype was rescued by the re-expression of STING1 (Figures 1K and 1L). penny sheldon raj